JAM2 ELISA Kit

Produktnummer ABIN2043831

Dieses Human JAM2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human JAM2 zu quantifizieren.

Kurzübersicht für JAM2 ELISA Kit (ABIN2043831)

Target: JAM2 (Junctional Adhesion Molecule 2 (JAM2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Produktdetails JAM2 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Marke: BioAssay™

Analytische Methode: Quantitative

Sensitivität: 13.3 pg/mL

Bestandteile: A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A (green), 1x120 µL
E: Detection Reagent B (red), 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30X, 1x20 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: The Human Junctional Adhesion Molecule 2 (JAM2) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of JAM2 in human serum, plasma, tissue homogenates and other biological fluids.The Microtiter Strip provided in this kit has been pre-coated with an antibody specific to JAM2. Standards or samples are then added to the appropriate Microtiter Strip wells with a biotin-conjugated antibody specific to JAM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain JAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of JAM2 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu JAM2

Target: JAM2 (Junctional Adhesion Molecule 2 (JAM2))